Open AccessMODIFICATION OF URINARY CATHETERS USING ANTIMICROBIALS FROM STREPTOMYCES SP. ABK 07 FOR URINARY TRACT INFECTION RESISTANCE
Author(s) -
Angima Bichang’A Kingsley,
R. Usha
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i7.25502
Subject(s) - antimicrobial , biofilm , catheter , microbiology and biotechnology , antibiotics , pseudomonas aeruginosa , urinary catheter , staphylococcus aureus , candida albicans , urinary system , chemistry , medicine , surgery , bacteria , biology , genetics
Objective: The main aim of this study is to prevent biofilm formation by impregnating an antimicrobial on urinary catheter.Methods: Catheter segments were immersed in the antimicrobial compound for impregnation. After 2 h, the segments were removed, sterilized and dried after which mechanical and antimicrobial properties of the catheter segments were determined. The shelf life of the impregnated segments was also ascertained as well as anti-biofilm assay. Spectral analysis (UV & FTIR) was also performed.Results: Impregnation was achieved by immersing catheter segments in antimicrobial compound ensuring it does not affect the catheter texture. The impregnated antimicrobial catheters were able to prevent colonization by common uropathogens Escherichia coli, Proteus, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella, and Candida albicans for up to 12 weeks. Antibiotic impregnation of the catheters did not affect the mechanical properties and did not render it as unfit for insertion. The antimicrobial-impregnated catheter offers a means of reducing biofilm formation and subsequently reducing the infection in long-term urinary catheter users. Spectral analysis was done by UV-Vis and FTIR.Conclusion: Antibiotic impregnation of the catheters did not affect the mechanical properties and did not render it as unfit for insertion. The antimicrobial impregnated catheter offers a means of reducing biofilm formation and subsequently reducing the infection in long-term urinary catheter users.