Open AccessIN VITRO STUDY OF ANTITRYPANOSOMAL ACTIVITY OF ETHANOLIC LEAF EXTRACT OF GARCINIA KOLA AGAINST TRYPANOSOMA BRUCEI BRUCEI
Author(s) -
A. H. B. Sani,
Umar Abdullahi Zakariyya,
Asmau Mahe,
Dilip K. Singh
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i6.25022
Subject(s) - trypanosoma brucei , incubation , garcinia kola , in vitro , traditional medicine , biology , glycoside , anthraquinones , parasite hosting , chemistry , botany , biochemistry , microbiology and biotechnology , medicine , gene , world wide web , computer science
Objective: The study was aimed at evaluating in vitro antitrypanosomal activity of Garcinia kola against Trypanosoma brucei brucei.Methods: The plant materials were extracted and screened for phytochemicals. The antitrypanosomal activity of 70% and 100% G. kola extracts against the parasite was determined in vitro.Results: Both extracts contained saponins, tannins, carbohydrates, cardiac glycosides, and flavonoids. However, anthraquinones and alkaloids were undetected. The parasites were seen to be actively motile within the first 30 min post-incubation period in 70% hydroethanolic G. kola extract and were found dead at 40–60 min in a higher concentration of extract. After 10 min post-incubation of the parasites in 100% ethanolic extract of G. kola, the parasites were observed to be active at lower concentration but at higher concentrations of the extracts, no trypanosomes were seen.Conclusion: 100% extract induces ceasing of motility at the lesser time compared to 70% ethanolic extract. Similarly, the effect increases with increase in extract concentration. Further research should be carried out to elucidate the bioactive compounds present to have a broad knowledge on the mode of action of the compounds.