Open AccessANTIMUTAGENIC EFFECTS OF ETHANOL EXTRACT OF SYZYGIUM MYRTIFOLIUM WALP. IN CYCLOPHOSPHAMIDE-INDUCED MICE
Author(s) -
Irene Sondang Lingga,
Urip Harahap,
Yuandani Yuandani
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i5.24350
Subject(s) - cyclophosphamide , micronucleus , ethanol , chemistry , micronucleus test , hematocrit , positive control , pharmacology , traditional medicine , toxicity , biology , biochemistry , medicine , endocrinology , chemotherapy , organic chemistry
Objective: This current study was carried to evaluate the antimutagenic effects of ethanol extract Syzygium myrtifolium Walp. in cyclophosphamide-induced mice.Methods: The mice were divided into 6 groups with the total number of 30 mice (CMC Na 0,5%; cyclophosphamide 30 mg/kg bw; and extract 50, 100, 200, and 400 mg/kg bw) consisting of 5 rats for each group. All mice were induced by cyclophosphamide 30 mg/kg bw on day 1, then followed by administration of extract for 7 days. On day 8, the blood was collected to evaluate the hematocrit value, while the femoral bone marrow was collected to determine the number of micronucleus cells.Results: The ethanol extracts of S. Myrtifolium leaves at doses of 50–400 mg/kg bw demonstrated antimutagenic effect by inhibiting the formation of micronucleus as compared to negative control (CMC Na 0.5%). Meanwhile, the hematocrit value increased at dose of 100, 200, and 400 mg/kg bw as compared to negative control.Conclusion: This finding suggests that ethanol extract of S. myrtifolium has antimutagenic activity that may increase the normal cell number in cyclophosphamide-induced mice.