Open AccessANALYSIS REACTIVITY OF PUNICA GRANATUM POLYPHENOLS TO THE OSTEOCALCIN, BONE MORPHOGENETIC PROTEIN-2, AND COLLAGEN TYPE-1
Author(s) -
Edrizal Burhan,
Bergman Thahar,
Trimurni Abidin,
Deddi Prima Putra,
Basri A. Gani
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i12.29683
Subject(s) - osteocalcin , chemistry , punica , reactivity (psychology) , polyphenol , ethyl acetate , biochemistry , alkaline phosphatase , enzyme , medicine , traditional medicine , antioxidant , pathology , alternative medicine
Objective: Punica granatum (PG) contains anthocyanins which are useful as antioxidants, anti-inflammatory, and prevent cancer, while also increasing bone cell proliferation and osteoblasts differentiation in bone remodeling.Methods: The reactivity of osteocalcin protein markers with PG polyphenol fractionation hydrogels observed using enzyme-linked immunosorbent assay, the degree of reactivity was determined by optical density at 550 nm.Results: PG butanol fraction has better reactivity compared to the total extract, ethyl, and hexane fraction. Based on the reactivity distribution, bone morphogenetic protein (BMP)-2 and collagen Type-1 had a dominant distribution compared to osteocalcin, but the theses proteins had a strong relation (r = 0.8) with probability (P < 0.05).Conclusion: PG butanol fraction had better reactivity to osteocalcin, BMP-2, and collagen Type-1 compared with total extract, hexane, and ethyl fraction. The four PG polyphenol fractionations have dominant reactivity to BMP-2.