Open AccessEFFECTS OF ANESTHESIA ON INNATE IMMUNE COMPONENTS IN ORTHOPEDIC SURGERY
Author(s) -
Adnan Hamad Aubaid,
Khalid Lahmood Yassen
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i12.29554
Subject(s) - medicine , cd16 , white blood cell , flow cytometry , tumor necrosis factor alpha , innate immune system , immunology , monocyte , eosinophil , basophil , immune system , gastroenterology , anesthesia , antibody , cd8 , immunoglobulin e , cd3 , asthma
Objectives: The present study was carried out to evaluate the possible role of anesthetics and surgery on innate by measuring the levels of tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ) by enzyme-linked immunosorbent assay (ELISA). Flow cytometry was used to determine MCP-I and CD16.Methods: Serum level of TNFα and IFNγ cytokines was measured using ELISA technique. Flow cytometry was used to determine MCP-I and CD16. White blood cell count and C-reactive protein (CRP) were determined.Results: There was a highly significant rise in CRP blood level postoperatively when compared with pre-operative blood level (p 0.05).