Open AccessOPTIMUM CONDITIONS FOR N-ACETYL GLUCOSAMINE PRODUCTION FROM TIGER SHRIMP (PENAEUS MONODON) SHELL BY SERRATIA MARCESCENS
Author(s) -
Yuniwaty Halim,
Hardoko Hardoko,
Ratna Handayani,
Vania Lucida
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i12.28956
Subject(s) - penaeus monodon , serratia marcescens , shrimp , glucosamine , chitin , incubation , incubation period , fermentation , food science , biology , microbiology and biotechnology , chemistry , zoology , biochemistry , fishery , chitosan , escherichia coli , gene
Objective: The aim of this research was to determine the optimum condition for Serratia marcescens to produce optimum amount of N-acetyl glucosamine using chitin isolated from tiger shrimp (Penaeus monodon) shells.Methods: This research was conducted using submerged fermentation method. The treatments used were various fermentation temperatures (20, 30, and 37°C), pH (6, 7, and 8), and incubation period (2, 4, 6, and 8 days).Results: Chitinolytic index of Serratia marcescens was 2.203±0.59 after 2 days of incubation. Optimum temperature for N-acetyl glucosamine production using S. marcescens was at 30°C. Optimum pH and incubation period for N-acetyl glucosamine production were at pH 8 and 6 days of incubation period.Conclusion: S. marcescens is able to ferment chitin from shrimp shell to produce N-acetyl glucosamine of 41,166.11±4,480.59 mg/l at optimum fermentation condition.