Open AccessTHE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC® 6514™ IN VITRO
Author(s) -
Ameta Primasari,
Minasari Nasution,
Nurul Hidayati Arbi,
Dini Permata Sari,
Mohammad Basyuni
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i12.28435
Subject(s) - minimum inhibitory concentration , minimum bactericidal concentration , subculture (biology) , traditional medicine , agar dilution method , aggregatibacter actinomycetemcomitans , hand sanitizer , agar , antibacterial activity , significant difference , negative control , serial dilution , chemistry , microbiology and biotechnology , food science , in vitro , medicine , biology , bacteria , biochemistry , dentistry , periodontitis , genetics , porphyromonas gingivalis , alternative medicine , pathology
Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC® 6514™ growth.Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCC® 6514 ™ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskal–Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.Results: Concentration of MIC from soursop leaf extract on Aa ATCC® 6514™ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCC® 6514™ from each treatment group.Conclusion: Soursop leaf extract has antibacterial effectivity against Aa ATCC® 6514 ™.