Open AccessP-SELECTIN GLYCOPROTEIN LIGAND-1 GENE POLYMORPHISMS IN SUDANESE PREGNANT WOMEN WITH THROMBOSIS
Author(s) -
A El-Hassan,
Fathelrahman Mahdi Hassan,
Hanan B. Eltahir
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i12.28200
Subject(s) - genotyping , genotype , thrombosis , medicine , polymorphism (computer science) , polymerase chain reaction , pregnancy , allele , gene , gastroenterology , immunology , obstetrics , genetics , biology
Objectives: In this study we aimed to investigate whether P-selectin gene polymorphism is associated with thrombosis in Sudanese pregnant women in Khartoum state.Methods: After informed consent, 96 Sudanese pregnant women with thrombosis and 53 healthy pregnant women were recruited in the study. Genomic DNA was isolated from whole blood. Genotyping of PSGL-1 gene was performed by polymerase chain reaction amplification for exon 14 and electrophoresis.Results: The frequency of the B allele was found to be significantly higher in pregnant women with thrombosis (26%) compared to the controls (17.4%).Conclusion: The AB genotype was found to be higher in women with thrombosis 40.6% than in healthy controls 20% (p=0.02). Our results suggest that the PSGL-1 AB genotype is associated with an increased risk of thrombosis in Sudanese pregnant women.