Open AccessTRANSFORMING GROWTH FACTOR BETA OF TESTIS GERMINAL CELL IN GUINEA PIG (CAVIA PORCELLUS) AFTER EXPOSURE TO METHANOL EXTRACT OF THE SEEDS OF BITTER MELON (MOMORDICA CHARANTIA) AND DEPOT MEDROXYPROGESTERONE ACETATE
Author(s) -
Syafruddin Ilyas,
Salomo Hutahaean,
Nursal Nursal
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i12.27920
Subject(s) - momordica , traditional medicine , melon , medroxyprogesterone acetate , biology , zoology , medicine , horticulture , endocrinology , estrogen
Objective: The discovery of male contraceptive drugs continues to be pursued, due to the very small participation of men associated with the lack of contraceptiveÐptions for men. The combination of methanol extract of bitter melon seed and depot medroxyprogesterone acetate (DMPA) becomes the choice that currently being pursued to be applied to men.Methods: The use of guinea pigs as experimental animals conducted research using experimental methods with complete randomized design. The study was divided into four control groups i.e K0; dimethylsulfoxide (DMSO) for 0 week (4 h),K1; Control group of DMSO for 4 weeks, K2; Control group of DMSO for 8 weeks, K3; Control group of DMSO for 12 weeks, and four treatment groups, i.e: group P0; bitter melon seed extract of 50 mg/100g body weight/day for 0 week (4 h), group P1; Bitter melon seed extract of 50 mg/100g BW/day for 4 weeks+DMPA, group P2; Bitter melon seed extract of 50 mg/100g BW/day for 8 weeks+DMPA, P3 group; Bitter melon seed extract of 50 mg/100g BW/day for 12 weeks+DMPA.Results: There was a significant effect (p<0.05) methanol extract of bitter melon seed to increase the transforming growth factor expression -β expression.Conclusion: The methanol extract of bitter melon seed was able to be candidate for herbal contraception.