Open AccessTHE EFFICACY OF 200 ΜG/KGBW/IP HEAT SHOCK PROTEIN-70 IN REDUCTION OF CYT C, BAX, AND CASPASE 3 EXPRESSION, AND MORTALITY IN MICE MODEL WITH MULTIPLE ORGAN DYSFUNCTION SYNDROME
Author(s) -
Igl Sukamto,
Suroto Suroto,
Ambar Mudigdo,
Bambang Purwanto
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i11.29541
Subject(s) - multiple organ dysfunction syndrome , apoptosis , lipopolysaccharide , sepsis , immunohistochemistry , hsp70 , medicine , andrology , pharmacology , septic shock , lung , heat shock protein , immunology , biology , biochemistry , gene
Objective: Heat shock protein 70 (HSP70) decreases Cyt expression c, Bax, and Caspase 3 in apoptosis multiple organ dysfunction syndrome (MODS), thus inhibiting death. This study aimed to analyze the efficacy of HSP70 200 μg/KgBB/ip to decrease Cyt c, Bax, and Caspase 3 expression, to reduce mortality, and to increase survival rate, in the MOD alveolar lung epithelial of 78-h sepsis model.Methods: This was a post-test only quasi-experiment conducted at Inter-University Central Laboratory of Gadjah Mada University, Yogyakarta, and the Anatomy Pathology Laboratory, Faculty of Medicine, Universitas Sebelas Maret, Surakarta. The study used a type of Balb/c mice, male, aged of 6–8 weeks, body weight of 25–33 g. Sepsis induction used LIG SIGMA L2880-10MG Lot #025M4040V from Escherichia coli 055:B5 purified by phenol extraction. Medication to reduce mortality used HSP70 Lot #L16020515 and then continued with 400× immunohistochemistry (IHC) examination. A sample of 30 mice were divided into three groups: (1) Control group without 78 h treatment, (2) lipopolysaccharide (LPS) group with a dose of 0.25 mg/kgBW/ip 78 h, and (3) HSP70 group with a dose of 200 μg/kgBB/ip after LPS injection 0.25 mg/kgBW/ip 78 h. The outcome variables included expression of Cyt, Bax, Caspase 3, and mortality in mice model with multiple organ dysfunction syndrome. The data were analyzed by Kruskal–Wallis and continued by Mann–Whitney U-test. Results: Administration of HSP70 200 mg/KgBW/ip after LPS 0.25 mg/kgBW/ip significantly decreased Cyt c expression (p=0.014), Bax (p=0.004), and Caspase 3 (p=0.015) in 78 h pulmonary alveolar cells, reduced mortality rate, and increased the number of survivors. Expressions of Cyt c, Bax, and Caspase 3 of IHC 400× magnification had a near-normal image change.Conclusion: There is a decrease of Cyt c, Bax, and Caspase 3 expression in the MOD alveolar lung epithelial cells of the 78-h sepsis model mice, a decrease of mortality rate, and an increase of survival rate, and the image of IHC is almost normal.