Open AccessEVALUATION AND CHARACTERIZATION OF BIOACTIVE COMPOUND OF HAMELIA PATENS PLANT WITH THE GC-MS SPECTROSCOPY
Author(s) -
Jafra Bano,
Swapna Santra,
Ekta Menghani
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i11.27557
Subject(s) - squalene , phytochemical , chemistry , gas chromatography–mass spectrometry , gas chromatography , chromatography , pentadecanoic acid , methanol , mass spectrometry , fatty acid , organic chemistry , biochemistry
Objective: The aim of this study is to evaluate the bioactive compound present in the leaves, root, flower, and stem by gas chromatography (GC) analysis. Novel drugs are isolated from plants against human diseases.Methods: The plant parts (leaves, stem, flower, and root) were collected, washed, shade dried, and powdered, and the methanol extracts of all plant parts were prepared by Soxhlet reflux method. The methanolic extracts were analyzed for the identification of phytochemical compounds present in the Hamelia patens plant parts using GC-mass spectrometry (MS) matched by the National Institute of Standards And Technology-11 library and Willey 8 library.Results: The bioactive fraction on GC-MS analysis revealed a chromatogram showing highest peaks. Methanol extracts of H. patens leaf are found to possess a wide range of saturated and unsaturated aliphatic hydrocarbons and their isomers such as 2,3-dihydro-3,5-dihydroxy-6-methyl- 4H-pyran (1.77%), 1,3-propanediol, 2-ethyl-2-(hydroxymethyl) (3.06%), mome inositol (18.22%), pentadecanoic acid (1.66%), and squalene (11.47%). GC-MS analysis revealed chromatogram, showing that the highest peaks of H. patens stem are methyl salicylate (3.41%), 2-amino-9-(3,4- dihydroxy-5- hydroxymethyl) (9.53%), mome inositol (63.73%), and squalene (1.07%).Conclusion: Isolation of such bioactives and their use as therapeutic target is the prime motto of our research to isolate novel bioactive that can used as potential therapeutic agents.