Open AccessSpectrophotometric flow injection method for the determination of Bendiocarb insecticide in water samples using chromogenic reagent 2,4-dinitrophenylhydrazine
Author(s) -
Malik H. Alaloosh Alamri,
Sadeem Subhi Abed,
Abdul Kareem Jawad Ali
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i10.28478
Subject(s) - bendiocarb , chromatography , chemistry , chromogenic , detection limit , reagent , spectrophotometry , pesticide , malathion , organic chemistry , agronomy , biology
Objective: Bendiocarb (BEN) is an acutely toxic carbamate insecticide which is used in public places and agriculture. The present study describes a new, sensitive, and accurate flow injection analysis method for the determination of BEN in its pesticide formulations and water samples.Methods: The developed method is based on an alkaline hydrolysis of BEN in NaOH, and the resultant product was coupled with 2,4-dinitrophenylhdrazin in the presence of sodium periodate to form red-colored product which measured at 515 nm.Results: Under the optimum conditions established (sample volume 150 μL, flow rate 2 mL/min, with 75 cm reaction coil length) for spectrophotometric determination of Bendiocarb. Beer’s law is obeyed in the range of 1–150 μg/mL with a detection limit of 0.738 μg/mL, with average recovery of 100.737 and relative standard deviation % of 1.331%.Conclusion: The established method was successfully applied for the determination of BEN in pesticide formulation and real spiked water sample.