Open AccessDETERMINATION OF PHYTOCOMPONENTS IN ETHANOL EXTRACT OF BRASSICA OLERACEA - USING GAS CHROMATOGRAPHY–MASS SPECTROSCOPY TECHNIQUE
Author(s) -
M. Jayalakshmi,
Vanitha,
R. Sangeetha
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2018.v11i10.26857
Subject(s) - chemistry , chromatography , brassica oleracea , gas chromatography , stigmasterol , phytol , ethanol , mass spectrometry , gas chromatography–mass spectrometry , campesterol , solvent , extraction (chemistry) , soxhlet extractor , organic chemistry , botany , biochemistry , sterol , biology , cholesterol
Objectives: Bioactive components determined by plants are known to have a broad application in the medical field. The focus of this study is to recognize the phytochemicals in the ethanol extract of Brassica oleracea by gas chromatography–mass spectroscopy (GC–MS). Methods: B. oleracea was collected, dried, and powdered well. The extraction was done with the solvent ethanol. The extract was exposed to column of GC-MS-QP 2010 (SHIMADZU) column Db 30.0 (0.25 μm in diameter, 0.25 μm thick). Results: GC–MS result provides the chromatogram with different peaks obtained at a different retention time shows the presence of various biocompounds. Some of the identified bioactive compounds are n-hexadecanoic acid (12.99%), phytol (2.40%), Vitamin E (3.38%), tetratetracontane (2.15%), stigmasterol (2.03%), and isophytol. Conclusion: The GC–MS study of the ethanol extract of B. oleracea reveals the existence of many potential compounds that can be utilized in the pharmaceutical industry, including the use of anti-inflammatory, antiarthritic, anticoronary, and antidiabetic agents.