Open AccessMETHYL GALLATE FROM JIRINGA (ARCHIDENDRON JIRINGA) AND ANTIOXIDANT ACTIVITY.
Author(s) -
Misri Yanty Lubis,
Rikson Siburian,
Lamek Marpaung,
Partomuan Simanjuntak,
M. Pandapotan Nasution
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v11i1.21637
Subject(s) - methyl gallate , chemistry , antioxidant , methanol , ethyl acetate , phytochemical , propyl gallate , carbon 13 nmr , proton nmr , chromatography , organic chemistry , fractionation , nuclear chemistry , column chromatography , gallate , gallic acid , biochemistry
Objective: This research was study about phytochemical active phenolic compound from pods of jiringa (Archidendron jiringa (Jack) I. C. Nielsen) and it’s antioxidant.Methods: Pods of jiringa were air dried and macerated with methanol. Extract was evaporated using rotary evaporator, and then, crude extract dissolved with water and partitioned with ethyl acetate. Extracts were evaporated and partitioned again with methanol and n-hexane. Column and preparative chromatography used to separate pure compound. Pure compound as methyl gallate was identificated with data from nuclear magnetic resonances of proton H (1H NMR), NMR of carbon (13C NMR), and mass spectrometry (MS). Antioxidant activity of pure compound tested by 1,1-diphenyl-2-picrylhydrazyl method.Results: We found pure phenolic compound from pods of jiringa as methyl gallate that exists on fraction III-2, and it has high antioxidant activity with inhibition concentration 50 was 3.7576 μg/ml.Conclusions: Pods of jiringa contain of active phenolic compound as methyl gallate that has high antioxidant activity. Therefore, it can be used as a source of natural antioxidant.