Open AccessEFFECTS OF THEAFLAVINS ON GASTRIC CANCER CELL LINE
Author(s) -
Surmi Roy,
Arpita Saha,
Ananya Chatterjee,
Sirshendu Chatterjee,
Sonal Mazumder,
Sandip K. Bandyopadhyay
Publication year - 2018
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v11i1.21606
Subject(s) - apoptosis , acridine orange , ethidium bromide , chemistry , programmed cell death , reactive oxygen species , caspase , microbiology and biotechnology , cytochrome c , viability assay , western blot , cell culture , cell , flow cytometry , biology , biochemistry , dna , gene , genetics
Objective: The objective of this study was to evaluate the anticarcinogenic effects of theaflavins (TFs), component of black tea, on human gastric cancer cell line.Materials and Methods: Cell viability was assessed by WST-1 reagent. Acridine orange (AO) and ethidium bromide (EB) fluorescence staining method were used to detect apoptotic cells, if any, followed by flow cytometric detection. To evaluate the molecular basis for understanding the apoptotic pathway, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were measured. Pro- and anti-apoptotic protein and gene expressions were detected by western blot and real-time techniques.Results: TFs-treated AGS cell indicated apoptosis-mediated cell death. The presence of TFs induced the generation of ROS and which in turn changes the MMP with the elevated level of cyt c, activation of caspase-9, caspase-3, and PARP cleavage. Our study also showed that TFs treatment altered the Bax/Bcl2 ratio and upregulated the expression of p53.Conclusion: All these observations lead to the fact that TFs developed potential chemopreventive effects through the induction of apoptosis through mitochondria-mediated death cascade in the human gastric cancer cell.