Open AccessTHE EFFECT OF CASEIN PHOSPHOPEPTIDE-AMORPHOUS CALCIUM PHOSPHATE-PROPOLIS CHEWING GUM ON CALCIUM AND PHOSPHATE ION LEVELS IN CARIES-FREE SUBJECT’S SALIVA AND STREPTOCOCCUS MUTANS BIOFILM FORMATION
Author(s) -
Sri Angky Soekanto,
Gadiacanaparimita Ghrena Duhita,
Endang W. Bachtiar,
Muhamad Sahlan
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10s5.23125
Subject(s) - amorphous calcium phosphate , streptococcus mutans , chemistry , biofilm , saliva , calcium , casein , propolis , phosphate , chewing gum , food science , remineralisation , chromatography , nuclear chemistry , biochemistry , fluoride , bacteria , inorganic chemistry , biology , organic chemistry , genetics
Objective: The aim of this study is to analyze the amount of calcium and phosphate ion released by casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), which supports the remineralization and the effectiveness of propolis wax in suppressing the mass formation of Streptococcus mutans biofilm on caries-free subjects and to observe if these two active components are effective when combined into one substance and placed inside sugar-free chewing gum.Methods: Twenty-five samples of caries-free saliva before and after the mastication simulation (five concentrations of chewing gum) in vitro were observed to check for release of calcium and phosphate ions along with a biofilm assay. The release of calcium ions was detected using AAS, the release of phosphate ions was detected using spectrophotometry ultraviolet-visible, and the biofilm assay was detected using a crystal violet 96-well plate ELISA and was evaluated with an ELISA reader.Result: Chewing gum with a concentration of 0% prop + 5% CPP-ACP showed the highest release level of calcium ions (p 0.05) and is significant in suppressing the mass formation of the S. mutans biofilm (p<0.05).Conclusions: Mastication simulation of CPP-ACP-Propolis chewing gum can increase calcium and phosphate ion levels in caries-free saliva and decrease S. mutans biofilm mass formation; this can support remineralization and becomes a dental caries prevention alternative.