Open AccessALPHA-GLUCOSIDASE INHIBITING ACTIVITY FROM THE MOST ACTIVE FRACTION OF CALOPHYLLUM TOMENTOSUM, WIGHT LEAVES
Author(s) -
Berna Elya,
Nuraini Puspitasari,
Fasya Hadaina Maharani
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10s5.23101
Subject(s) - saponin , ethyl acetate , fraction (chemistry) , terpenoid , flavonoid , chemistry , traditional medicine , methanol , chromatography , pharmacognosy , fractionation , inhibitory postsynaptic potential , biological activity , biochemistry , in vitro , biology , organic chemistry , medicine , antioxidant , alternative medicine , neuroscience , pathology
Objective: The purpose of this study was to obtain the inhibitory concentration, 50% value from the most active fraction of methanolic extract of leaf Calophyllum tomentosum, Wight, and to identify the group of compounds contained in the most active fraction.Methods: The methanol extract was fractionated using the silica gel static phase, the n-hexane motion phase, ethyl acetate, and methanol enhanced by its polarity. Results: There were nine selected fractions tested in inhibitory activity using an enzyme activity inhibitory assay. Conclusions: The results showed the inhibition by the most active fraction is 51.288 μg/mL; this fraction contains of flavonoid, saponin, and terpenoid.