Open AccessMYCOSYNTHESIS OF SILVER NANOPARTICLES: CHARACTERIZATION, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY FROM PLEUROTUS FLORIDA (MONT) SINGER: A MACRO FUNGI
Author(s) -
Athira Ragunath,
Parveen Nisha,
R. Kumuthakalavalli
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i9.19090
Subject(s) - dpph , antioxidant , chemistry , absorbance , silver nanoparticle , nuclear chemistry , ic50 , oxygen radical absorbance capacity , silver nitrate , antibacterial activity , pleurotus , in vivo , nanoparticle , food science , chromatography , biochemistry , materials science , in vitro , nanotechnology , biology , bacteria , microbiology and biotechnology , mushroom , genetics
Objective: To determine the antioxidant and anti-inflammatory activity of myco-synthesized silver nanoparticle (AgNP) from Pleurotus florida. Methods: Fresh mushrooms were collected, processed, and the AgNPs were synthesized using standard reducing agent 1 mM of silver nitrate. The characteristics of synthesized particles were confirmed using ultraviolet, Fourier transmission infrared, X-ray diffraction, and energy dispersive X-ray analysis. These nanoparticles were subjected for antioxidant activity through 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and anti-inflammatory activity using bovine serum albumin denaturation assay, respectively, and the percentage of inhibition of AgNPs was calculated with standard error mean.Results: The AgNPs were recorded the absorbance band at 410 nm was the particle size of 20-50 nm with a rod in shape. In DPPH assay the antioxidant activity was recorded as 96.2% with 50% inhibitory concentration (IC50) value of 85 μg/ml. In anti-inflammatory activity, the activity was recorded as 92.10±0.005% and IC50 value was recorded as 100 μg/ml.Conclusion: The results indicated the significant antioxidant and anti-inflammatory activity of AgNPs from P. florida. Hence, further in vivo studies are focused to confirm the anticancer and anti-inflammatory activities.