Open AccessSTRUCTURAL ELUCIDATION OF BIOACTIVE SECONDARY METABOLITES FROM ENDOPHYTIC FUNGUS
Author(s) -
Shiv Kumar Verma,
Madan Lal,
Mira Das
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i7.18909
Subject(s) - microbiology and biotechnology , biology , antimicrobial , pathogenic bacteria , aspergillus niger , klebsiella pneumonia , bacillus subtilis , proteus vulgaris , plant use of endophytic fungi in defense , antibacterial activity , bacteria , staphylococcus aureus , sclerotium , botany , biochemistry , genetics
Objective: In this research for bioactive secondary metabolites from endophytic fungal isolate CPR5, which was later identified and characterized as Aspergillus niger sp.Methods: Eight bioactive compounds (1-8) were isolated and purified using a series of chromatographic techniques and identified by applying 13C and 1H nuclear magnetic resonance and mass (ESI-MS spectrometry).Results: All the compounds were tested for antimicrobial activity against a wide range of human and plant pathogenic bacteria as well as fungi. These microorganisms were, Xanthomonas oryzae, Escherichia coli, Streptococcus pneumonia, Bacillus subtilis, Staphylococcus hyicus, Bacillus sphaericus, Staphylococcus aureus, and Pseudomonas aeruginosa, Candida albicans, Sclerotium rolfsii, Sclerotinia, scleratiourum, and Phoma exigua minimum inhibitory concentration value have also been determine for these microorganism.Conclusion: In this study, a total 10 endophytic fungi were isolated from the Calotropis procera, show a great antibacterial activity against 24 human pathogenic bacteria.