Open AccessOXIDATIVE STRESS REDUCING AND LIVER PROTECTIVE EFFICACY OF ETHYL ACETATE FRACTION ISOLATED FROM PAJANELIA LONGIFOLIA (WILLD) K. SCHUMAN
Author(s) -
Suparna Datta,
Manabendra Dutta Choudhury
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i6.16898
Subject(s) - chemistry , carbon tetrachloride , antioxidant , oxidative stress , glutathione , superoxide dismutase , lipid peroxidation , ethyl acetate , transaminase , catalase , glutathione peroxidase , pharmacology , biochemistry , bilirubin , alkaline phosphatase , enzyme , endocrinology , biology , organic chemistry
Objectives: We investigated the liver protective as well as oxidative stress reducing activity of isolated fractions (Pajanelia fraction-1, 2, and 3 [PF1, PF2, and [PF3]) from ethyl acetate extract of Indian medicinal plant Pajanelia longifolia (Willd.) K. Schuman.Methods: The liver protective activity of fractions was evaluated against carbon tetrachloride (CCl4) (0.5 ml/kg body weight intraperitoneal [b.w. i.p.]) induced hepatic damage in Swiss albino mice. Isolated fractions (at dose level of 150 mg/kg b.w. per orally [p.o.]) were administered to mice before 30 minutes of CCl4 administration. Silymarin at a dose of 50 mg/kg b.w. p.o. was taken as a standard drug. The liver protective activity of fractions was measured on serum biochemical parameters such as serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, serum alkaline phosphatase, and bilirubin (total). Further antioxidant activity of fractions was also measured on antioxidant enzymatic and non-enzymatic levels such as reduced glutathione, lipid peroxidation, superoxide dismutase, catalase, and glutathione peroxidase.Results: The in vivo study revealed that the PF3 most effectively protected the liver from damage by CCl4. The findings were also confirmed by histopathological observations. High-performance liquid chromatography result was clearly indicated that the fraction was pure.Conclution: Ethyl acetate fraction (Pajanelia fraction 3) from P. longifolia may be considered as a liver protective agent.