Open AccessMethod development and Validation of RP-HPLC method for the Determination of Olanzapine in Bulk and Tablet Dosage form
Author(s) -
S. V. Saibaba,
Shanmuga Pandiyan P
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i5.17375
Subject(s) - chromatography , olanzapine , relative standard deviation , dosage form , calibration curve , high performance liquid chromatography , methanol , phosphate buffered saline , chemistry , potassium , volumetric flow rate , materials science , analytical chemistry (journal) , detection limit , medicine , schizophrenia (object oriented programming) , organic chemistry , physics , quantum mechanics , psychiatry
Objective: To develop and validate reverse phase-high performance liquid chromatographic method for estimation of olanzapine in bulk and tablet dosage form.Methods: Chromatographic analysis was performed on XTerra C18 (150×3.5 mm inner diameter, 5 μm) column using a mobile phase consisting of buffer (potassium dihydrogen phosphate) and methanol (45:55% v/v) with a flow rate of 0.6 ml/minutes. The detection was carried out at 247 nm.Results: The calibration curve of olanzapine was linear in the range of 30-70 μg/ml. The mean % assay of marketed formulation was found to be 100.2%, and % recovery was observed in the range of 98-102%. Relative standard deviation for the precision study was found <2%.Conclusion: The developed method is simple, precise and rapid, making it suitable for estimation of olanzapine in bulk and tablet dosage form.Keywords: Olanzapine, Reverse phase-high performance liquid chromatographic, Validation.