ANTIHYPERTENSIVE ACTIVITY OF EXTRACT AND FRACTIONS OF MATOA (POMETIA PINNATA J. R & G FORTS) LEAVES

Objective: The purpose of this study was to determine antihypertensive activity of extract and fractions of matoa (Pometia pinnata) leaves.Methods: Matoa leaves were extracted by reflux, followed by evaporating using rotary evaporator. Hypertension was induced by 50 mg/kg bw. NaCland 1.5 mg/kg bw. prednisone orally, every day as long as 28 days, then continued over the next 28 days in the therapy period. Male Sprague Dawleyrats were divided into 12 groups which were hydrochlorothiazide (0.45 mg/kg bw.), control group hypertensive, control normal, matoa leaves extract(MLE) (with doses of 50 mg/kg bw., 100 mg/kg bw., and 150 mg/kg bw.), ethylacetate fraction (with doses of 4.35 mg/kg bw., 8.71 mg/kg bw., and13.06 mg/kg bw.), and water fraction (with doses of 10 mg/kg bw., 21.88 mg/kg bw., and 32.82 mg/kg bw.). Measurement of systolic and diastolicblood pressure was done every weeks using direct tail-cuff of noninvasive method. Then histomorphology of muscle heart was performed at the endof this research.Results: Ethylacetate fraction of matoa leaves 13.08 mg/kg bw. and MLE 150 mg/kg bw. gave significant result in lowering blood pressure (p<0.05) onthe 28th day of therapy and showed an equal profile with hydrochlorothiazide (0.45 mg/kg bw). Histomorphological result of rat’s muscle heart foundcollagen production was increased in NaCl-prednisone induced rats.Conclusions: Extract and fractions of P. pinnata leaves could decrease blood pressure of NaCl-prednisone induced hypertension rats, but this effectwas not liniear with doses and they did not decrease the collagen production in cardiac myocardium compared to normal group.Keywords: Pometia pinnata, Leaves, Hypertension, Tail-cuff noninvasive method, Blood pressure.