Open AccessPROTECTIVE EFFECT OF BEE PROPOLIS AGAINST ANTI-TUBERCULOSIS DRUGS (RIFAMPICIN AND ISONIAZID)-INDUCED HEMATOLOGICAL TOXICITY IN SPRAGUE DAWLEY RATS
Author(s) -
Umesh Bharti,
Neelima R. Kumar,
Jaspreet Kaur
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i3.15991
Subject(s) - propolis , isoniazid , rifampicin , medicine , pharmacology , toxicity , body weight , tuberculosis , traditional medicine , pathology
Objective: Protective effect of bee propolis against anti-tuberculosis (TB) drugs (rifampicin and isoniazid)-induced hematological toxicity in SpragueDawley (SD) rats.Methods: Experimental male SD rats weighing 180±20 g were randomly assigned into eight groups (n=6), the Group 1 served as control; Group 2received 200 mg bee propolis/kg body weight; Groups 3, 5, and 7 were treated with drugs 100 mg rifampicin/kg body weight, 50 mg isoniazid/kgbody weight, and 100 mg rifampicin+50 mg isoniazid/kg body weight, respectively. Groups 4, 6, and 8 were treatment groups receiving 200 mg beepropolis/kg body weight+100 mg rifampicin/kg body weight, 200 mg bee propolis/kg body weight+50 mg isoniazid/kg body weight, and 200 mg beepropolis/kg body weight+100 mg rifampicin+50 mg isoniazid/kg body weight, respectively. All the treatments were given for 30 days, and then, therats were sacrificed under light esthesia by cervical dislocation and blood was collected for physiological studies.Results: Bee propolis supplementation (200 mg/kg body weight) showed increased level of hemoglobin with respect to rifampicin (15.45%),isoniazid (11.34%), and rifampicin plus isoniazid (5.04%) administered groups after 30 days of treatment. Moreover, the decreased level of red bloodcell count and white blood cell count by anti-TB drugs rifampicin, isoniazid, and rifampicin plus isoniazid together was also elevated in treatmentgroup with bee propolis.Conclusion: Coadministration of propolis (200 mg bee propolis/kg body weight) with drugs helped modulate the toxic effects by restoring testedvalues to near normal.Keywords: Propolis, Rifampicin, Isoniazid, Hemoglobin, Red blood cell count, White blood cell count.