Open AccessEVALUATION OF ANTIBACTERIAL ACTIVITY OF INDONESIAN VARIETIES SWEET POTATO LEAVES EXTRACT FROM CILEMBU AGAINST Shigella dysenteriae ATCC 13313
Author(s) -
Sri Agung Fitri Kusuma
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i2.15773
Subject(s) - shigella dysenteriae , phytochemical , antibacterial activity , agar diffusion test , traditional medicine , antimicrobial , food science , shigella , minimum bactericidal concentration , tannin , minimum inhibitory concentration , chemistry , biology , botany , bacteria , microbiology and biotechnology , escherichia coli , salmonella , biochemistry , medicine , genetics , gene
Objective: The aim of this study was to evaluate the in Vitro antibacterial activity of sweet potato (Ipomoea batatas (L.) Lam) leaves ethanol extract against Shigella dysenteriae ATCC 13313.Methods: The study was performed by collecting and determining plant samples, extraction, moisture content assay, phytochemical screening, Thin layer Chromatography, antibacterial activity using agar diffusion method using perforator of 11 mm in diameter and Minimum Inhibition Concentration (MIC) test using macrodilution method against Shigella dysenteriae ATCC 13313.Results: The Phytochemical analysis of the sweet potato leaves extract revealed the presence of flavonoids, tannin, steroid and polyphenolic compound. The sweet potato leaves ethanol extract showed significant zone of inhibition in a dose dependent manner against Shigella dysenteriae ATCC 13313 with the range of MIC and MBC was 10-20 % b/v.Conclusion : It can be concluded that the sweet potato leaves ethanol extract shows promise as an antibacterial agent for inhibiting bacillary dysentery due to the presence of biologically active ingredients with antimicrobial activity in the extract.Keywords: sweet potato, Cilembu, Indonesia, antidysentery, Shigella dysenteriae ATCC 13313