Open AccessSUPPLEMENTATION WITH 2:1 RATIO OF N-6:N-3 POLYUNSATURATED FATTY ACID IMPROVES LIVER STEATOSIS AND SERUM CYTOKINE LEVELS IN YOUNG OBESE BALINESE WOMEN: A RANDOMIZED CLINICAL TRIAL
Author(s) -
I Wayan Weta,
Tjokorda Gde Bagus Mahadewa,
Wayan Putu Sutirtayasa,
Anak Agung Ngurah Subawa,
Safarina G. Malik,
I Putu Eka Widyadharma
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i12.20851
Subject(s) - medicine , polyunsaturated fatty acid , odds ratio , nonalcoholic fatty liver disease , triglyceride , confidence interval , steatosis , body mass index , obesity , fatty liver , linoleic acid , gastroenterology , endocrinology , fatty acid , cholesterol , biochemistry , disease , chemistry
Objectives: In addition to the rise in obesity prevalence globally, morbidity due to nonalcoholic fatty liver disease is increasing. Primary modalities for preventing and managing this problem include dietary modification and improved physical activities. A daily diet with a low n-6:n-3 polyunsaturated fatty acid (PUFA) ratio is suspected to contribute to ameliorating liver steatosis (LS). The present study was conducted to elucidate the effects of an n-6:n-3 PUFA ratio of 2:1 in alleviating LS.Methods: Twenty-four young obese women with LS were recruited from Denpasar, Bali, Indonesia. They were randomly allocated to an intervention or control group. Both groups were given linoleic acid:α-linolenic acid at ratios of 2035:970 and 240:100 g, respectively, for 12 weeks. Baseline and end-line data were obtained. All patients were advised to maintain their daily energy intake no more than 1500 kcal and to perform structured physical exercises once a week.Results: The intervention significantly decreased the body fat (body mass index, p=0.040; triglyceride, p=0.008) and serum tumor necrosis factor-α (TNF-α) levels (p=0.002) and increased serum interleukin-10 (IL-10) levels (p=0.004). The severity of LS was reduced through the intervention (odds ratio=0.064; 95% confidence interval=0.013-0.310; p=0.001).Conclusion: An increased intake of 2:1 n-6:n-3 PUFA ratio alleviated LS, decreased body fat composition and serum TNF-α levels, and increased serum IL-10 levels.