Open AccessEFFECT OF ETHANOLIC EXTRACT OF CYANOTIS CRISTATA LEAVES APPLIED TOPICALLY ON WOUND HEALING IN WISTAR RATS
Author(s) -
Anurag Pathak,
Smita Shenoy,
Sushil Kiran,
Avinash Aujayeb,
Deepak M Nayak,
Naveen Kumar,
Amod Tilak,
Manjunath Shetty
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i12.20731
Subject(s) - wound healing , breaking strength , burn wound , granulation tissue , medicine , silver sulfadiazine , surgery , analysis of variance , traditional medicine , materials science , composite material
Objective: The objective of this study was to assess the effect of ethanolic extract of Cyanotis cristata leaves when applied topically on healing of wound in Wistar rats.Methods: C. cristata leaves were evaluated for its effect on healing of wound in three models, namely, incision, excision, and burn wound. Each model included five groups, each consisting of 6 rats. The five groups were as follows: Group I rats (control and ointment base), Group II rats (standard and silver sulfadiazine), and Group III, Group IV, Group V (treated with C. cristata extract ointment of 0.5%, 1%, and 2%, respectively). Variables evaluated included breaking strength in incision wound while it was contraction rate and epithelialization in excision and burn wound. One-way analysis of variance and Tukey’s post hoc test was used to analyze data.Result: In incision wound, breaking strength in test group was significantly increased (p<0.001) as compared to control. In excision and burn wound of test group, time to epithelialization and contraction rate was significantly decreased (p<0.001). The granulation tissue from excision and burn wound showed increased collagen and less inflammatory cells in test groups in comparison to the control.Conclusion: The ethanolic extract of leaves of C. cristata, when applied topically, enhanced wound strength and accelerated healing of incision, excision, and burn wounds in rats.