Open AccessTHE INVESTIGATION OF CONDITIONS OF API FROM GROUP OF CALCIUM CHANNEL BLOCKERS EXTRACTION BY ORGANIC SOLVENTS BY USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AS ASSAY METHOD
Author(s) -
Olgya Polyauk,
Liliya Logoyda
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i10.20506
Subject(s) - nifedipine , verapamil , amlodipine , chemistry , chromatography , extraction (chemistry) , calcium channel , hexane , chloroform , calcium , pharmacology , organic chemistry , medicine , blood pressure
Objective: The objective of this research was to select the optimal conditions for extraction of API from calcium channel blockers (amlodipine, nifedipine, and verapamil) by organic solvents from water solutions in dependence on pH solutions.Methods: The chromatographic analysis of amlodipine, nifedipine, and verapamil performed on liquid chromatography Agilent 1200.Results: The quantitative determination of API from calcium channel blockers (amlodipine, nifedipine, and verapamil) by high-performance liquid chromatography - method has been conducted. As a result of studies, we have found that the optimal extragent is chloroform, which is extracted at pH 7-82.1% of amlodipine, pH 5-76.2% of nifedipine, and pH 8-95.1% verapamil hydrochloride. API from calcium channel blockers (amlodipine, nifedipine, and verapamil) least extracted with hexane at pH 2.0, so these conditions may be cleaned extract from coextractives impurities.Conclusion: The results obtained in this research work clearly indicated that the extraction of API from calcium channel blockers (amlodipine, nifedipine, and verapamil) by organic solvents from water solutions in dependence on pH solutions has been conducted.