Open AccessGAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS, IN VITRO CYTOTOXIC AND ANTIOXIDANT EFFICACY STUDIES ON CLEOME GYNANDRA L. (LEAVES): A TRADITIONAL DRUG SOURCE
Author(s) -
Renuka Saravanan,
P. Brindha,
Mahesh Narayanan,
Sivakumar Ramalingam
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i10.19343
Subject(s) - ethyl acetate , dpph , phytochemical , chemistry , antioxidant , chromatography , gas chromatography–mass spectrometry , traditional medicine , phenols , mass spectrometry , organic chemistry , biochemistry , medicine
Objective: This study was aimed to assess the phytoconstituents, cytotoxic, and antioxidant efficacy of ethyl extract of Cleome gynandra leaves.Methods: Qualitative phytochemical analysis with different solvent extracts was performed. Quantitative and gas chromatography-mass spectrometry (GC-MS) analysis of the extract was performed with ethyl acetate extract. The cytotoxic effect of the ethyl acetate extract was determined by 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) assay on Michigan Cancer Foundation-7 (MCF-7) cells using taxol as standard and free radical scavenging ability using 1,1-diphenyl-2-picrylhydrazyl (DPPH).Results: Leaves extracts with different solvents revealed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, and tannins. GC-MS analysis of ethyl acetate of the plant leaves showed the presence of n-hexadecanoic acid. The IC50 value of the ethyl acetate extract was found to be 90.2 μg/ml on MCF-7 cell line, and the extract was found to possess significant DPPH free radical scavenging activity.Conclusion: From the results, we conclude that the C. gynandra extract possesses antioxidant and antiproliferating activity against MCF-7 cells.