Open AccessTHE EFFECT OF ALPHA-MANGOSTIN IN BALANCING THE RATIO OF CYTOKINES PRO-AND ANTI-INFLAMMATION-GAMMA (IFN-γ/IL-10) AND SEVERITY OF THE DISEASE IN MICE INFECTED WITH MYCOBACTERIUM TUBERCULOSIS MULTIDRUG-RESISTANT
Author(s) -
Dyan Kunthi Nugrahaeni,
Suharyo Hadisaputro,
Ari Suwondo,
Edi Dharmana
Publication year - 2016
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2016.v9s3.14544
Subject(s) - tuberculosis , medicine , mycobacterium tuberculosis , immunology , interferon gamma , cytokine , multiple drug resistance , gastroenterology , pharmacology , drug resistance , microbiology and biotechnology , biology , pathology
Objective: The objective of this study was observed to measure the effect of alpha-mangostin in balancing the ratio of interferon-gamma (IFN-γ) andinterleukin-10 (IL-10), and the severity of the disease in mice which infected with Mycobacterium tuberculosis multidrug-resistant (TB-MDR).Method: Infected BALB/c mice were consisted of five groups: Treated with anti-TB drugs+α-mangostin, treated with anti-TB drugs, given α-mangostinduring treatment, and control group. Cytokine levels of culture supernatant of spleen cells were measured by enzyme-linked immunosorbent assay.The number of bacterial colonies was derived from a primary cell culture of bronchoalveolar lavage. Statistical analysis was performed with Anova,Kruskal-Wallis test and correlation Pearson, and Spearman-rank test.Result: Median IFN-γ production was higher in mice, which given with α-mangostin during treatment is 1838.2 pg/ml and control is 1585.5 pg/mlcompared treated with anti-TB drugs+α-mangostin (1312 pg/ml) and anti-TB drugs (1429.3 pg/ml) (p>0.05). The highest result production of medianIL-10 in the 3th group is (465.91 pg/ml) and the lowest in the control group is 195.29 pg/ml, p>0.05. Median IFN-γ/IL-10 ratio of the 3th group verylow (3.94), it means the 3th group is experienced with severity of TB. Alpha-mangostin was decreased in severity of disease based on the number ofTB-MDR bacterial colonies (p≤0.05).Conclusion: α-mangostin have an effect on the balancing IFN-γ/IL-10 ratio and reduce a severity of TB-MDR with using immunomodulator.Keywords: Tuberculosis multidrug-resistant, Alpha-mangostin, Interferon-γ/interleukin-10 ratio, Number of tuberculosis multidrug-resistantbacterial colonies.