Open AccessISOLATION AND CHARACTERIZATION OF N-HEPTACOSANYL OLEATE FROM THE WHOLE AERIAL PARTS OF CENTELLA ASIATICA LINN.
Author(s) -
Rashmi Saxena Pal,
Yogendra Pal,
Pranay Wal,
Vijender Singh
Publication year - 2016
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2016.v9s3.13427
Subject(s) - centella , phytochemical , chromatography , chemistry , petroleum ether , column chromatography , methanol , traditional medicine , organic chemistry , extraction (chemistry) , medicine , biochemistry
Various studies have already been performed involving the whole aerial parts of Centella asiatica (L.) (Umbelliferae), commonly known as gotu kola orjalbrahmi, and thus, the present investigation has been carried out for the phytochemical study of an ethanolic extract of the aerial parts of C. asiatica.To perform this activity, the drug (1.5 kg) was exhaustively extracted in 95% ethanol using Soxhlet apparatus. The column chromatography wasperformed then for isolating the various phytoconstituents using the solvents of increasing polarity from petroleum ether to methanol. The isolatedcompounds were structurally elucidated using various spectral data analysis, i.e. infrared,1H nuclear magnetic resonance (NMR),13C NMR, and positiveion fast atom bombardment mass spectrometry. One of the isolated compounds was characterized as n-heptacosanyl oleate.Keywords: Whole aerial parts, Centella asiatica, Soxhlet, Column, Heptacosanyl oleate.