Open AccessPHYTOCHEMICAL CONTENT AND ANTIOXIDANT ACTIVITIES IN DIFFERENT ORGANS OF POMELO (CITRUS MAXIMA [BURM.] MERR.) USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL AND PHOSPHOMOLYBDENUM ASSAYS
Author(s) -
Irda Fidrianny,
Elvira Sari,
Komar Ruslan
Publication year - 2016
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2016.v9s2.13526
Subject(s) - chemistry , dpph , flavonoid , antioxidant , phytochemical , food science , carotenoid , nuclear chemistry , biochemistry
Objectives: The aims of this research were to determine antioxidant activity from various organs extracts of pomelo using 2,2-diphenyl-1picrylhydrazyl (DPPH) and phosphomolybdenum assays, total phenolic, flavonoid, and carotenoid content, correlation of total phenolic, flavonoid, and carotenoid content in various extracts of chayote with their inhibitory concentration 50% (IC ) of DPPH antioxidant activities and exhibitory concentration 50% (EC 50 ) of phosphomolybdenum capacity, and correlation between two antioxidant assays. 50 Methods: Extraction was carried out by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities using DPPH and phosphomolybdenum assays, determination of total phenolic, flavonoid, and carotenoid content were conducted by UVvisible spectrophotometry and its correlation with IC Results: The lowest IC 50 50 of DPPH and EC 50 of phosphomolybdenum was analyzed by Pearson’s method. of DPPH scavenging activity was shown by ethyl acetate cortex extract of pomelo (0.68 µg/ml), whereas the lowest EC of phosphomolybdenum capacity was given by ethyl acetate leaves extract of pomelo (101.36 µg/ml). Ethyl acetate cortex extract of pomelo had the highest total phenolic content and ethyl acetate leaves extract had the highest total flavonoid content (TFC). There was a negative and significant correlation between TFC in cortex and peel extracts of pomelo with their IC 50 of DPPH. Conclusions: All organs extracts of pomelo (except n-hexane peel extract) were classified as a very strong antioxidant by DPPH method. Flavonoid compounds in cortex and peel extract of pomelo were the major contributor in antioxidant activities by DPPH method. DPPH and phosphomolybdenum assays gave no linear results in antioxidant activities of leaves, cortex, and peel extracts of pomelo. Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Phosphomolybdenum, Pomelo, Three organs. 50