Open AccessPHYTOCONSTITUENTS FROM RICHARDIA SCABRA L. AND ITS BIOLOGICAL ACTIVITIES
Author(s) -
Poonkodi Kathirvel
Publication year - 2016
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2016.v9i6.14015
Subject(s) - petroleum ether , hela , oleic acid , cytotoxicity , chemistry , ether , methanol , chromatography , extraction (chemistry) , biochemistry , botany , cell , biology , organic chemistry , in vitro
Objective: The present chemical investigation is carried out for the isolation of active phytoconstituents from Richardia scabra Linn belongs to Rubiaceae family.Methods: Air dried plant material was macerated with petroleum ether and defatted plant was introduced in Soxhlet apparatus with methanol. Both extracts were subjected to column chromatography for isolation of active chemicals.Result: Two novel coumarins from the methanol extract and one fatty acid from the petroleum ether extract were isolated from Richardia. Oleic acid, heraclenin and isopsoralen were isolated for the first time in the genus Richardia. Furthermore, the cytotoxicity of the heraclenin was evaluated with HeLa cell line and cell cycle arrest by Fluorescent assisted cell sorting analysis.Conclusion: The result revealed that the heraclenin has potent cytotoxicity with the IC50 value was 46.09µg/ml and it induced cell cycle arrest in G0/G1 phase about (51.2%), S phase (30.2) and resulted in a decline in the percentage of G2-M phase.Keywords: cell cycle, cytotoxicity, Heraclenin, isopsoralen, Oleic acid, Richardia scabra