Open AccessTHE EFFECT OF CLIMATE ON TOTAL PHENOLICS IN MACROTYLOMA UNIFLORUM, VIGNA UNGUICULATA, CINNAMOMUM ZEYLANICUM AND MENTHA PIPERITA USING SPECTROPHOTOMETER
Author(s) -
Vasundhara Saxena,
Chagti Kk
Publication year - 2016
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2016.v9i5.11643
Subject(s) - vigna , cinnamomum zeylanicum , tannin , polyphenol , chemistry , proanthocyanidin , botany , cinnamomum , horticulture , traditional medicine , biology , food science , antioxidant , essential oil , medicine , biochemistry , alternative medicine , traditional chinese medicine , pathology , cassia
Objective: Polyphenolic compound which is proven as antioxidants and possesses a chemo - protective potential are found in leaves, flowers, fruits,and bark. The aim of the study was to estimate the amount of tannins present in these drugs using Folin-Denis method. This study was conductedto evaluate the variations in the total amount of secondary metabolites (poly-phenolic) during summer and winter seasons in the leaves of fourimportant valuable medicinal plants, viz., Macrotyloma uniflorum, Vigna unguiculata, Cinnamomum zeylanicum, and Mentha piperita using ultravioletvisiblespectrophotometry.Methods: Tannin contents of plants were measured by Folin-Denis method. Tannin-like compounds reduce phosphotungstomolybdic acid in alkalinesolution to produce a highly colored blue solution, the intensity of which is proportional to the amount of tannins present. The intensity is measuredin a spectrophotometer at 650 nm.Results: The concentration was almost same for Mu1 and Mu2 plant with little effect of seasonal variations and at the same place it was found to bemaximum in Cz1. The concentration curve for tannic acid was determined, and the correlation coefficient was calculated and was found to be 1 whichindicates the good linearity between the concentration and the absorbance.Conclusion: The maximum amounts of secondary metabolites (poly-phenolic) were observed during summer, while minimum in winter season withan exception of Mu2 plant. Thus, this study was used as one of the parameters for standardization of medicinal plants.Keywords: Macrotyloma uniflorum, Vigna unguiculata, Cinnamomum zeylanicum, Mentha piperita tannins, Total phenolics, Folin-Denis method.