Open AccessCryopreservation of Hopea odorata Roxb. Seed : Effect of Cryoprotectan Treatment on Physiological and Biochemical Change
Author(s) -
Laila Ainur Rohmah,
Dian Latifah,
Fitri Fatma Wardani,
Aulia Hasan Widjaya,
Kumala Dewi
Publication year - 2022
Publication title -
journal of tropical biodiversity and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2540-9581
pISSN - 2540-9573
DOI - 10.22146/jtbb.67360
Subject(s) - germination , cryoprotectant , cryopreservation , malondialdehyde , horticulture , botany , chemistry , biology , food science , biochemistry , oxidative stress , embryo , microbiology and biotechnology
Hopea odorata Roxb. is a forest plant from Dipterocarpaceae family that play a crusial economic and ecological functions. Its propagation from generative materials is limited because of the recalcitrant seed. The aim of this study is to evaluate the effect of cryoprotectant and cryopreservation treatment as an alternative seed storage technique on the physiological and biochemical change of H. odorata seeds. Fresh seeds were treated with two cryoprotectan, PVS1 (mannitol) and PVS2 (DMSO 15% (w/v), ethylene glycol 15% (w/v) and glycerol 30% (w/v). in 0.4 M sucrose) with different concentration (25, 50, 75 and 100% (w/v)) and immersion time (30, 60, 90 and 120 min). Seeds were stored at room temperature (28±2⁰C) for evaluation of cryoprotectant toxicity and in liquid nitrogen (-196±2⁰C) for 24 hours. In room temperature storage, seed were immersion into 100%, 75% and 50% of PVS1 showed higher germination value (germination percentage, germination rate, vigour index, maximum growth potensial) and lower biochemical accumulation (electrolyte leakage, total malondialdehyde, total phenol) than PVS2. Cryoprotectant and cryopreservation treatments in this study was not suitable to increase the viability of H. odorata seed. Seeds were unable to germinate cause tissue necrosis and increase of total malondialdehyde.