Open AccessCytotoxicity of ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) on HeLa cell line
Author(s) -
Adisty Ridha Damasuri,
Eti Nurwening Sholikhah,
. Mustofa
Publication year - 2020
Publication title -
indonesian journal of pharmacology and therapy
Language(s) - English
Resource type - Journals
ISSN - 2745-455X
DOI - 10.22146/ijpther.606
Subject(s) - hela , cytotoxicity , chalcone , cytotoxic t cell , mtt assay , cell culture , ic50 , microbiology and biotechnology , chemistry , biology , stereochemistry , in vitro , biochemistry , genetics
In our previous study, some amino chalcone derivatives have been synthesized and evaluated their cytotoxicity against breast cancer cell line T47D. Among 11 amino chalcone derivatives, ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) exhibited the most active compound.This study aimed to investigate cytotoxic activity of the ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) against cervical cell line (HeLa). The cytotoxic activitywas determined using the MTT colorimetric assay. Cisplatin was used as positive control. From this MTT method, inhibitory concentration 50% (IC50) values were determined by probit analysis based on the relationship between log concentrations versus the percentage of cells growth inhibition.The results showed that the IC50 of ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) and cisplatinwere 22.75 ± 19.13 μg/mL and 14.96±1.08 μg/mL, respectively. In conclusion, the ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) has moderate cytotoxic activity against HeLa cell line based on National Cancer Institute (NCI) criteria.