Open AccessEffect of Isolation Method on Characteristics of Microcrystalline Cellulose from Brown Seaweed Sargassum vulgare
Author(s) -
Amir Husni,
Budhiyanti Siti Ari
Publication year - 2022
Publication title -
indonesian journal of pharmacy
Language(s) - English
Resource type - Journals
eISSN - 2338-9486
pISSN - 2338-9427
DOI - 10.22146/ijp.3274
Subject(s) - microcrystalline cellulose , hydrolysis , crystallinity , solubility , cellulose , residue (chemistry) , chemistry , enzymatic hydrolysis , acid hydrolysis , nuclear chemistry , chromatography , food science , organic chemistry , crystallography
Microcrystalline cellulose (MCC) has been reported to be purely derived from depolymerized cellulose which forms crystals. Therefore, this study aims to determine the characteristics of MCC samples which were in form of powder and alginate residues of Sargassum vulgare, hydrolyzed with acid and enzymes. The observed characteristics included yield, water content, ash content, pH, solubility, functional groups, and crystallinity index. Furthermore, the results showed that the MCC from acid-hydrolyzed seaweed powder and alginate residue, as well as enzymatically hydrolyzed seaweed powder and alginate residue each, had a yield of 3.83±2.74%, 5.66±1.52%, 10.03±2.58%, and 17.17±3.50%, crystallinity index of 91.37%, 80.26%, 84.67%, and 81.90%, water content of 4.92±1.85%, 4.92±1.11%, 3.88±2.01%, and 3.58±0.40%, ash content of 13.88±0.12%, 3.49±0.13%, 9.86±0.17%, and 7.43±0.09%, pH of 5.13±0.38, 5±0.1; 5.7±0.17, and 5.97±0.06, and solubility of 22.82±1.20%, 23.73±1.09%, 19.12±3.55%, and 21.10±1.48%, respectively. The functional group analysis showed that there were similarities with the standard Avicel PH101. Also, water content and pH meet the requirements according to the Handbook of Pharmaceutical Excipients, while solubility and ash content does not meet these requirements. However, the results of enzymatic hydrolysis were better than acid hydrolysis, and samples from alginate residues had better results than seaweed powder. Considering these results, the best MCC was obtained from enzymatically hydrolyzed alginate residue with a yield, water content, ash content, pH, solubility, and crystallinity index of 17.17±3.50%, 3.58±0.40, 7.43±0.09, 5.97±0.06, 21.10±1.48, and 81.90%.