MONOLITHIC DISK FOR THE FAST CHROMATOGRAPHIC SEPARATION | Zendy

Nurul Hidayat Aprilita | Zendy; Rania Bakry | Zendy; Christian W. Huck | Zendy; Guenther K. Bonn | Zendy

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MONOLITHIC DISK FOR THE FAST CHROMATOGRAPHIC SEPARATION

Author(s) -

Nurul Hidayat Aprilita,

Rania Bakry,

Christian W. Huck,

Guenther K. Bonn

Publication year - 2010

Publication title -

indonesian journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.273

H-Index - 14

eISSN - 2460-1578

pISSN - 1411-9420

DOI - 10.22146/ijc.21815

Subject(s) - divinylbenzene , chemistry , styrene , tetrahydrofuran , copolymer , chromatography , acetonitrile , phase (matter) , analytical chemistry (journal) , organic chemistry , polymer , solvent

Poly(styrene/divinylbenzene) (PS/DVB) monolithic disk was prepared by in situ free-radical copolymerization of styrene and divinylbenzene in the presence of decanol and tetrahydrofuran as porogens. PS/DVB monolithic disks were produced in two different lengths 1.5 mm and 3 mm. The disks were used in reversed phase chromatography of proteins with 0.2 % trifuoroacetic acid (TFA) and 0.2 % TFA in acetonitrile as mobile phase A and B, respectively. The effect of gradient rate, flow rate, temperature and disk length on the separation of proteins were also studied. PS/DVB monolithic disks allow the rapid separation of proteins in reversed phase chromatography.Keywords: monolithic disk, poly(styrene/divinylbenzene), proteins

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