Open AccessMONOLITHIC DISK FOR THE FAST CHROMATOGRAPHIC SEPARATION
Author(s) -
Nurul Hidayat Aprilita,
Rania Bakry,
Christian Huck,
Guenther K. Bonn
Publication year - 2010
Publication title -
indonesian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.273
H-Index - 14
eISSN - 2460-1578
pISSN - 1411-9420
DOI - 10.22146/ijc.21815
Subject(s) - divinylbenzene , chemistry , styrene , tetrahydrofuran , copolymer , chromatography , acetonitrile , phase (matter) , analytical chemistry (journal) , organic chemistry , polymer , solvent
Poly(styrene/divinylbenzene) (PS/DVB) monolithic disk was prepared by in situ free-radical copolymerization of styrene and divinylbenzene in the presence of decanol and tetrahydrofuran as porogens. PS/DVB monolithic disks were produced in two different lengths 1.5 mm and 3 mm. The disks were used in reversed phase chromatography of proteins with 0.2 % trifuoroacetic acid (TFA) and 0.2 % TFA in acetonitrile as mobile phase A and B, respectively. The effect of gradient rate, flow rate, temperature and disk length on the separation of proteins were also studied. PS/DVB monolithic disks allow the rapid separation of proteins in reversed phase chromatography.Keywords: monolithic disk, poly(styrene/divinylbenzene), proteins