Open AccessPRODUCTION AND CHARACTERIZATION OF BIOSURFACTANT BY <i>Pseudomonas fluorescens</i> USING CASSAVA FLOUR WASTEWATER AS MEDIA
Author(s) -
Venty Suryanti,
Soerya Dewi Marliyana,
Desi Suci Handayani,
Desi Ratnaningrum
Publication year - 2013
Publication title -
indonesian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.273
H-Index - 14
eISSN - 2460-1578
pISSN - 1411-9420
DOI - 10.22146/ijc.21281
Subject(s) - chemistry , critical micelle concentration , kerosene , wastewater , food science , emulsion , pseudomonas fluorescens , soybean oil , nuclear chemistry , micelle , organic chemistry , bacteria , waste management , biology , aqueous solution , engineering , genetics
Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flourescens using cassava flour wastewater (manipueira) as media. The ability of P. flourescens to produce biosurfactant could suggest potential use in industrial and environmental applications. Media containing a mixture of natural manipueira and nutrient broth with 48 h fermentation was the optimum condition for the biosurfactant production. Based on UV-Vis and FT-IR spectra, the biosurfactant was indicated as rhamnolipids containing hydroxyl, ester, carboxylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC) value of 715 mg/L and reduced the surface tension of the water from 80 mN/m to 59 mN/m. The biosurfactant was able to decrease the interfacial tension about 51-70% when benzyl chloride, palm oil and kerosene were used as water-immiscible compounds. The biosurfactant was able to form stable emulsion until 30 days when paraffin, soybean oil, lubricant oil and kerosene were used as water-immiscible compounds.