Open AccessImmunological aspects of bacterial keratites in patients with diabetes mellitus
Author(s) -
О.В. Заволока,
Павло Андрійович Бездітко,
Л. П. Абрамова,
V. O. Vekshyn
Publication year - 2022
Publication title -
arhìv oftalʹmologìï ukraïni
Language(s) - English
Resource type - Journals
eISSN - 2311-2999
pISSN - 2309-8147
DOI - 10.22141/2309-8147.9.2.2021.241501
Subject(s) - keratitis , medicine , diabetes mellitus , ophthalmology , cornea , cytokine , gastroenterology , endocrinology
Background. The purpose was to analyze the cytokine balance of lacrimal fluid in patients with bacterial keratitis and diabetes mellitus (DM) at the first visit and to identify the immunological aspects of the disease. Materials and methods. The analysis of pro- and anti-inflammatory cytokine concentration in the lacrimal fluid was performed in 17 patients with type 1 DM and bacterial keratitis and 15 nondiabetic patients with bacterial keratitis at the first visit. Data from 14 healthy individuals were used for comparison. In addition to standard ones, ophthalmic examination methods included bacteriological examination, fluorescein test, anterior segment optical coherence tomography, non-contact corneal aesthesiometry. The levels of interleukin (IL) 1β, IL-6 and IL-10 in the lacrimal fluid of the sick and the contralateral eye were determined by a quantitative colorimetric enzyme-linked immunosorbent assay using ELISA kits. Results. In DM patients with bacterial keratitis, the concentration of IL-1β and IL-6 in the lacrimal fluid of the sick eye exceeded that in healthy individuals (p 0.05). In the lacrimal fluid of the contralateral eye of DM patients with bacterial keratitis, the level of IL-1β and IL-6 exceeded the corresponding indicators of nondiabetic patients with bacterial keratitis and healthy individuals (p 0.05). Conclusions. DM patients with bacterial keratitis have immunological features of the disease.