Open AccessInflammatory response of stem cell secreting conditioned media in SH-SY5Y cell line
Author(s) -
Faizan Ahmad
Publication year - 2020
Publication title -
bionatura
Language(s) - English
Resource type - Journals
eISSN - 1390-9355
pISSN - 1390-9347
DOI - 10.21931/rb/2020.05.03.13
Subject(s) - stem cell , mesenchymal stem cell , sh sy5y , inflammation , inflammatory response , cell culture , immunology , cell , secretion , proinflammatory cytokine , biology , cancer research , microbiology and biotechnology , medicine , neuroblastoma , endocrinology , biochemistry , genetics
Mesenchymal stem cells (MSCs) are reported to secrete anti-inflammatory cytokines and growth factors, which makes MSCs a promising candidate in the treatment of various neurodegenerative diseases. SH-SY5Y show extreme inflammatory response under LPS and an inadequate inflammatory response when treated with Wharton's jelly, conditioned media. This study mainly focuses on the inflammatory (pro and anti-inflammatory) response of SH-SY5Y by gene expression study. SH-SY5Y cell line used for cell culture and RT- q PCR was done with 5 different primers. In this article, lipopolysaccharides (LPS) show a significant result in pro-inflammatory and pro-apoptotic. In this article, we focus on the therapeutic approach of stem cells, which reduce inflammation by secreting stem cell factors to cure various neurodegenerative diseases.