Open AccessThe search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study)
Author(s) -
Rosa Gagliardi B,
Diana Martínez A,
Beatriz Tellechea H,
Pedro Sitjar Q,
Silvia Llambí D,
María Arruga L
Publication year - 2015
Publication title -
revista mvz córdoba/revista mvz cordoba
Language(s) - English
Resource type - Journals
eISSN - 1909-0544
pISSN - 0122-0268
DOI - 10.21897/rmvz.78
Subject(s) - genotyping , biology , gene , mutation , subfamily , genetics , genotype , gene mutation , microbiology and biotechnology
Objective. The objective of this study is to analyze the frequency of mdr1-1Δ mutation in German Shepherd, Doberman, Border Collie and Greyhound dog breeds in Uruguay. Materials and methods. A total of 95 animals from the four breeds mentioned above were studied. DNA was isolated from blood using potassium acetate with a subsequent degradation from RNA with RNAsaH. The concentration and quality of the DNA obtained was evaluated with a Nanodrop, ND-1000 spectrophotometer. To determine the presence or absence of the mdr1-1Δ mutation, DNA samples were sent to Gene Seek, Neogen Corporation of Chicago, United States, for genotyping. Results. In all 95 animals studied, the mdr1-1Δ mutation was not present. Conclusions. Based on the preliminary results obtained, other elements that may cause adverse drug reactions must be considered: unidentified mutations in other regions of the MDR1 gene; mutations in other genes involved in the transport of drugs from the same subfamily or another; mutations in enzymes involved in drug metabolism (e.g. Cytochrome P450). Moreover, especially with Border Collies and Greyhounds, it is advisable to increase the number of animals in the study.Key words: Dogs, ivermectine, P-glycoprotein (Source: CAB).