Open AccessConcordance between otic cytology and culture in diagnosis of external otitis canine by Malassezia spp
Author(s) -
Adriana Pulido-Villamarín,
Rubiela Castañeda-Salazar,
Melva Yomary Linares-Linares,
Marcela Mercado-Reyes
Publication year - 2015
Publication title -
revista mvz córdoba/revista mvz cordoba
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.202
H-Index - 11
eISSN - 1909-0544
pISSN - 0122-0268
DOI - 10.21897/rmvz.42
Subject(s) - concordance , cytology , malassezia , otitis , kappa , medicine , dermatology , biology , pathology , surgery , linguistics , philosophy
ABSTRACT Objective. To determine the correlation between microbiological culture and otic cytology for diagnoses of external otitis by Malassezia in dogs. Materials and methods. 158 ear swabs of dogs with clinical diagnosis of external otitis were analyzed by cytology, mycological culture and metabolic tests. Results. Were obtained a positive results by cytology of 62% and 75.3% by culture. The 31.1% of isolates were identified as M. pachydermatis, 12.6% as M. furfur and 56.3% were classified as Malassezia spp., because was not possible to define the species. We found a positive concordance between cytology and culture for Malassezia spp., of 0.76 with a kappa index of 0.448 (95% CI 0.30 to 0.60) which represents a moderate strength of concordance between the two techniques, without regard the identified species. Conclusions. The use of a diagnostic test is not enough to establish the participation of Malassezia spp., as a causal disease agent.