Open AccessGenetic Screening of Deficiency of Uridine Monophosphate Synthase in Holstein Freisian Crossbred Cattle
Author(s) -
Sherly Ignetious,
Shrikant Joshi,
Ranjit Aich,
Shefali Macwan
Publication year - 2017
Publication title -
the indian journal of veterinary sciences and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2395-1176
pISSN - 2394-0247
DOI - 10.21887/ijvsbt.v13i02.10051
Subject(s) - microbiology and biotechnology , biology , gene , genotype , enzyme , restriction fragment length polymorphism , allele , genetics , biochemistry
Fifty Holstein Friesian (H.F) crossbredcattle were screened for Deficiency of Uridine MonophosphateSynthase (DUMPS) using PCR-RFLP. Blood samples were collected from jugular veins in 2 mlcapacity vaccutainers containing 2 mg/ml (K2 EDTA). DNA was isolated from the blood samples byusing whole blood extraction kit. PCR was performed for amplification of polymorphic region ofUridine Monophosphate Synthase (UMPS) gene (108 bp) on bovine chromosome 1. The PCRproducts of 108 bp were digested with AvaI endonuclease enzyme. The normal allele in unaffectedcattle produced three fragments of 53 bp, 36 bp and 19 bp. No animal was found carrier for UMPSgene. The genotype frequency of normal individuals and the gene frequency of normal allele werefound to be one.