Open AccessMotion Characteristics and Kinematics of Fresh Spermatozoa of Gir, Surti and Murrah Bulls Assessed By Computer Assisted Semen Analyzer
Author(s) -
A. J. Dhami,
P.K. Pathak,
D. V. Chaudhari,
K. K. Hadiya
Publication year - 2018
Publication title -
the indian journal of veterinary sciences and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2395-1176
pISSN - 2394-0247
DOI - 10.21887/ijvsbt.14.3.4
Subject(s) - murrah buffalo , semen , sperm , biology , breed , zoology , extender , veterinary medicine , anatomy , chemistry , medicine , organic chemistry , polyurethane , botany
A study was carried out on semen of nine breeding bulls, three each of Gir, Surti and Murrah breeds, to evaluate the comparative motion characteristics and kinematics of their fresh spermatozoa by CASA. The ejaculates (n=72, 24 of each breed)having >75% initial motility were diluted @ 80 million sperm/ml using TFYG extender and were assessed for motion characteristics by CASA. The overall mean values of rapid motile and immotile sperm per cent were observed significantly greater inGir bulls semen, while total motile and slow motile sperms were apparently higher in buffalo semen. The mean values of sperm velocity/ kinematic parameters observed based on all motile sperms in Gir, Surti and Murrah bulls semen were: average pathvelocity 50.01±1.25, 48.51±1.03 and 49.14±1.30 μm/s; curvilinear velocity 88.62±1.66, 87.90 ±1.74 amd 88.93±1.69 μm/s, straight line velocity 44.51±1.35, 43.14±1.12 amd 41.73±2.24 μm/s; linearity 50.06±1.42, 48.86±1.32 amd 48.49±1.84 %; straightness85.17±0.92, 84.97±0.88 and 83.90±1.17 %; wobbling index 57.00±1.17, 55.32±1.05 and 55.30±1.48 %; beat-cross frequency 15.55±0.58, 16.14±0.43 and 14.97±0.54 hz; amplitude of lateral head displacement 2.39±0.18, 2.57±0.12 and 2.31±0.14 μm; dancingfrequency 208.34±15.52,225.00 ±10.74 and 211.29±13.03 μm2/s, and dancing mean 5.70±0.46, 6.33±0.35 and 6.33±0.50 μm2/s, respectively. Almost similar trend with little higher values were noted for sperm velocity/kinematics based on only progressivelymotile sperm, without breed/species variation. The semen of all three breeds behaved identically for sperm kinematics. The bull variation was insignificant for all the traits in all the three breeds.