Open AccessIsolation, Molecular Detection and Phylogenetic analysis of Avipox Virus obtained from Pigeon
Author(s) -
J. John Kirubaharan,
Ranjani Rajasekaran,
P. Shilpa,
M. Vidhya,
S. Rajalakshmi
Publication year - 2018
Publication title -
the indian journal of veterinary sciences and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2395-1176
pISSN - 2394-0247
DOI - 10.21887/ijvsbt.14.3.10
Subject(s) - embryonated , phylogenetic tree , biology , virus , virology , gene , polymerase chain reaction , inoculation , isolation (microbiology) , virus isolation , dna extraction , genetic analysis , microbiology and biotechnology , genetics , immunology
Dried scab material obtained from pigeon showing cutaneous form of avipox infection was inoculated in chorio-allantoic membrane (CAM) of 11-day old embryonated chicken egg. After an incubation of five days, CAM showed mild pock lesions. ThisCAM tissue was subjected to DNA isolation. Molecular confirmation of avipox virus was done by PCR amplification of P4b gene, which amplified at an expected product size of 578 bp. The amplified P4b gene was sequenced and phylogenetic analysis wasdone along with P4b gene sequences of other avipox viruses. Phylogenetic analysis showed that this avipox virus obtained from pigeon was grouped under sub-clade A2 along with other pigeonpox viruses. This confirmed that the causative agent of theavipox infection observed in pigeon was pigeonpox virus.