Open AccessBasophil activation test in the diagnosis of allergic reactions to local anesthetics
Author(s) -
O. N. Chibisova,
G. I. Lugovskaya,
O. V. Khabarova
Publication year - 2022
Publication title -
medicinskij vestnik ûga rossii
Language(s) - English
Resource type - Journals
eISSN - 2618-7876
pISSN - 2219-8075
DOI - 10.21886/2219-8075-2022-13-1-124-128
Subject(s) - basophil activation , medicine , immune system , sensitization , immunology , hypersensitivity reaction , drug , immunoglobulin e , pharmacology , basophil , anesthesia , antibody
Objective: to evaluate the detection of in vitro hypersensitivity to local anesthesia drugs by fl ow cytometry. Materials and methods : in the test of activation of basophils, the determination of the hypersensitivity reaction to local anesthetics of groups I, II (154 preparations) in 106 patients and T-lymphocytes of type 2 immune response (CD3 + CD294 +) was carried out by fl ow cytometry using the Allergenicity kit test system. In the history of the examined patients, there was a certain food and/ or drug intolerance. Results: Th e levels of sensitization to diff erent groups of local anesthetics were compared in the basophil activation test. Th e highest levels of hypersensitivity were observed to local anesthetics containing vasoconstrictors, stabilizers. In patients with hypersensitivity to drugs for local anesthesia, an increase in the number of T-lymphocytes of type 2 of the immune response was observed. Conclusions: based on the identifi ed hypersensitivity in the basophil activation test to local anesthesia drugs, the safest anesthetics were established. Th e diagnostic signifi cance of determining the level of T-lymphocytes of type 2 of the immune response in drug hypersensitivity is shown.