Open AccessМетод количественного описания фиксированных гистологических образцов с помощью цифровой голографической микроскопии
Author(s) -
А. В. Белашов,
А.А. Жихорева
Publication year - 2021
Publication title -
pisʹma v žurnal tehničeskoj fiziki
Language(s) - English
Resource type - Journals
eISSN - 1726-7471
pISSN - 0320-0116
DOI - 10.21883/pjtf.2021.09.50901.18641
Subject(s) - digital image analysis , microscopy , digital holographic microscopy , quantitative analysis (chemistry) , computer science , digital holography , characterization (materials science) , pathology , holography , biomedical engineering , artificial intelligence , materials science , computer vision , chemistry , optics , medicine , physics , chromatography , nanotechnology
A novel method for the quantitative characterization of fixed histological samples based on the statistical analysis of their phase images obtained using digital holographic microscopy is developed and presented. The proposed approach allows for fully automated processing of reconstructed phase images and obtaining quantitative data of morphological and optical characteristics of histological tissues structures. The method was validated on three histological samples of different types of tissues: ciliated columnar epithelium, elastic cartilage, and liver.