Open AccessПоляризованная флуоресценция молекул NADH при двухфотонном возбуждении фемтосекундными лазерными импульсами с длиной волны 720-780 nm
Author(s) -
М.Э. Сасин,
И.А. Горбунова,
Н.O. Безверхний,
Я.М. Бельтюков,
J. RubayoSoneira,
О.С. Васютинский
Publication year - 2019
Publication title -
pisʹma v žurnal tehničeskoj fiziki
Language(s) - English
Resource type - Journals
eISSN - 1726-7471
pISSN - 0320-0116
DOI - 10.21883/pjtf.2019.13.47956.17803
Subject(s) - fluorescence , spectroscopy , femtosecond , chemistry , fluorescence correlation spectroscopy , aqueous solution , fluorescence spectroscopy , excitation , rotational diffusion , analytical chemistry (journal) , nicotinamide adenine dinucleotide , laser , photochemistry , materials science , nad+ kinase , optics , physics , molecule , organic chemistry , chromatography , quantum mechanics , biochemistry , enzyme
The decay of polarized fluorescence in coenzyme NADH upon two-photon excitation by femtosecond laser pulses at the wavelength range of 720- 780 nm in water/methanol solution has been investigated. Decay fluorescence times tau_1 and tau_2, rotation diffusion time tau_rot, and preexponential factors a_1/a_2 ratio have been determined from experiment. The results obtained have been compared with previous results in NADH in aqueous solution. polarized fluorescence, NADH, adenine, nicotinamide, spectroscopy, time correlation single photon spectroscopy