Open AccessТемпературная зависимость кинетики затухания флуоресценции производных тиофлавина T в глицерине
Author(s) -
В.И. Степуро
Publication year - 2022
Publication title -
optika i spektroskopiâ
Language(s) - English
Resource type - Journals
eISSN - 2782-6694
pISSN - 0030-4034
DOI - 10.21883/os.2022.05.52423.13-22
Subject(s) - thioflavin , fluorescence , analytical chemistry (journal) , chemistry , kinetics , atmospheric temperature range , excited state , photon counting , glycerol , fluorescence spectroscopy , spectroscopy , materials science , thermodynamics , optics , chromatography , organic chemistry , atomic physics , photon , physics , medicine , disease , pathology , quantum mechanics , alzheimer's disease
Possible use of Thioflavin T based fluorescent molecular rotors as temperature sensors was assessed in this work. Fluorescent properties of Thioflavin T and its derivative 6-Me-BTA-2C in 99 % glycerol were studied using steady-state and time-resolved fluorescence spectroscopy methods. For Thioflavin T in glycerol it has been found that temperature growth from 261 K to 353 K results in ~3 orders of magnitude increase of non-radiative decay rate constant k_nr for the excited state of the molecule. Fluorescence decay studies by time-correlated single photon counting method showed that fluorescence decay kinetics of Thioflavin T in glycerol can be used for temperature measurements in the range 260–290 K. For 6-Me-BTA-2C molecule the range of the maximal sensitivity was shifted to higher temperatures (280–320 K). The obtained results about fluorescent properties and decay kinetics of Thioflavin T based dyes in highly viscous media can be used to develop nanoscale temperature sensors.