Open AccessМикроскопия с многофотонным возбуждением для идентификации и оперативного контроля компонентов внеклеточного матрикса тканей организма
Author(s) -
Ю В Кистенев,
В.В. Николаев,
А.В. Борисов,
О.Б. Заева,
А.И. Князькова,
Н.А. Кривова
Publication year - 2020
Publication title -
žurnal tehničeskoj fiziki
Language(s) - English
Resource type - Journals
eISSN - 1726-748X
pISSN - 0044-4642
DOI - 10.21883/os.2020.06.49412.50-20
Subject(s) - elastin , extracellular matrix , dermis , process (computing) , matrix (chemical analysis) , microbiology and biotechnology , cell , isolation (microbiology) , chemistry , pathology , anatomy , biology , biochemistry , computer science , medicine , chromatography , bioinformatics , operating system
The process of extraction of the extracellular matrix (ECM) requires operational monitoring since with non-optimal process parameters there is a risk of destruction of the basic proteins of the cell-matrix. Using multiphoton microscopy, the structure of collagen, the integrity of the cell membrane and the content of elastin in the process of isolation of ECM from various tissues of the rat organism (brain, dermis, muscle) were studied. It has been shown that multiphoton microscopy is an effective tool for monitoring the quality of ECM extraction.